Recipe

BAC Injection Buffer

Tris (pH 7.5)  10 mm
EDTA  0.1 mm
NaCl 100 mm
Adjust the pH to 7.4, and add H2O to 1 L, using water for embryo transfer (Sigma-Aldrich, catalog no. W1503). Filter the injection buffer (through a 0.2-U filter) and store at 4°C.
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  1. doi:10.1101/pdb.rec099481 Cold Spring Harb Protoc 2019: pdb.rec099481- © 2019 Cold Spring Harbor Laboratory Press
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