Recipe

Fusion Selection (HAT) Medium

Reagent Volume to add (mL) Manufacturer Catalog number Lot number Strength
DMEM 180 Cellgro 15-017-CM 15017099
Fetal bovine serum 75 HyClone SH30070.03 ATD 31955 ∼25%
Glutamax 3.9 GIBCO 35050 354496 100×
Pen/strep 3.9 GIBCO 15140 417319 100×
NCTC109 39 GIBCO 21340 621024
Gentamicin 0.3 Sigma-Aldrich G1397 908200
HAT 4 ATCC 69-X 58012860
Hybridoma Cloning Factor 9 R&D 206-IL 0JZ0408062R 3%
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  1. doi:10.1101/pdb.rec104208 Cold Spring Harb Protoc 2019: pdb.rec104208- © 2019 Cold Spring Harbor Laboratory Press
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