Pronase Solution (2×)
| Reagent | Quantity (for 20 mL) | Final concentration |
|---|---|---|
| Pronase (Roche) | 40 mg | 0.2% |
| Tris-Cl (1 m, pH 7.6) | 2 mL | 100 mm |
| EDTA (0.5 m, pH 8.0) | 0.08 mL | 2 mm |
| SDS (10%) | 2 mL | 1% |
| H2O | 15.9 mL | |
| Mix the solution well by vortexing and incubate for 45 min at 37°C for self-digestion and activation. Aliquot the ready-to-use 2× pronase solution into 1.5-mL Eppendorf tubes and store at −20°C. | ||
