Infection Medium
| Reagent | Quantity (for 1 L) | Final concentration |
|---|---|---|
| DMEM | 945 mL | |
| Fetal bovine serum (FBS) | 20 mL | 2% |
| HEPES (1 m, pH 8.0) | 25 mL | 25 mm |
| Penicillin–streptomycin (P/S) solution (100×) | 10 mL | 1× |
| Store at 4°C. | ||
| Reagent | Quantity (for 1 L) | Final concentration |
|---|---|---|
| DMEM | 945 mL | |
| Fetal bovine serum (FBS) | 20 mL | 2% |
| HEPES (1 m, pH 8.0) | 25 mL | 25 mm |
| Penicillin–streptomycin (P/S) solution (100×) | 10 mL | 1× |
| Store at 4°C. | ||