Supplementation Medium
| Reagent | Quantity (for 1 L) | Final concentration |
|---|---|---|
| F-12/K medium | 790 mL | |
| Fetal bovine serum (FBS) | 200 mL | 20% |
| Penicillin–streptomycin (P/S) solution (100×) | 10 mL | 1× |
| Store at 4°C. | ||
| Reagent | Quantity (for 1 L) | Final concentration |
|---|---|---|
| F-12/K medium | 790 mL | |
| Fetal bovine serum (FBS) | 200 mL | 20% |
| Penicillin–streptomycin (P/S) solution (100×) | 10 mL | 1× |
| Store at 4°C. | ||