Recipe

Supplementation Medium

Reagent Quantity (for 1 L) Final concentration
F-12/K medium 790 mL
Fetal bovine serum (FBS) 200 mL 20%
Penicillin–streptomycin (P/S) solution (100×)  10 mL  1×
Store at 4°C.
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  1. doi:10.1101/pdb.rec099598 Cold Spring Harb Protoc 2019: pdb.rec099598- © 2019 Cold Spring Harbor Laboratory Press

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