Buffer A for Antibody Cross-Linking (1×)
| Reagent | Volume per 500 mL of solution (v/v) | Final concentration |
|---|---|---|
| Tris–HCl (1 m, pH 8.0) | 25 mL | 50 mm |
| NaCl (5 m) | 15 mL | 150 mm |
| EDTA (0.5 m) | 5 mL | 5 mm |
| Nonidet P-40 (10% solution) | 5 mL | 0.1% |
| ddH2O | Add to 500 mL | |
| Freshly prepare a 10% Nonidet P-40 solution before addition to Buffer A. Store at 4°C. | ||
