Mounting Medium (with Anti-Fade Reagent)
| Reagent | Amount to add | Final concentration |
|---|---|---|
| Tris-HCl (1 m, pH 8.8) | 0.8 mL | 20 mm |
| p-phenylenediamine | 0.20 g | 0.5% |
| Glycerol (100%) | 36 mL | 90% |
| Mix the above ingredients together in a 50-mL conical tube. Adjust volume to 40 mL with H2O. Dissolve the solids by slowly bubbling nitrogen gas through a Pasteur pipette inserted deep into the mixture. Filter through a 0.8-µm filter at room temperature. Store at −20°C in 1- or 3-mL syringes devoid of air bubbles. Discard when dark brown. Handle this reagent with gloves; it causes stains when spilled. | ||
