Marc's Modified Ringers (MMR) (25×)
| Reagent | Amount to add | Final concentration (25×) | Final concentration (1×) |
|---|---|---|---|
| HEPES free acid | 119.2 g | 125 mm | 5 mm |
| NaCl | 584.5 g | 2.5 m | 100 mm |
| KCl | 14.91 g | 50 mm | 2 mm |
| MgCl2 | 20.33 g | 25 mm | 1 mm |
| CaCl2 | 29.4 g | 50 mm | 2 mm |
| EDTA (0.5 m, pH 8.0) | 20 mL | 2.5 mm | 0.1 mm |
| To prepare a 25× stock, dissolve the above ingredients in 3.8 L of H2O, and adjust the pH to pH 7.8 with 10 m NaOH. Bring the final volume to 4 L, and store at room temperature. | |||
