Preparation of Polymerase Chain Reaction Templates from Embryonic and Adult Mouse Tissue Samples
Abstract
A simple cell or tissue lysate can provide a sufficient quality and amount of template DNA for polymerase chain reaction (PCR). This protocol can be used to prepare embryonic tissues, yolk sac, ear punch, toe, or 1- to 2-mm (maximum 5 mm) tail samples for PCR analysis.
Footnotes
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From the Manipulating the Mouse Embryo collection, edited by Richard Behringer, Marina Gertsenstein, Kristina Vintersten Nagy, and Andras Nagy.
