Preparation of dsRNAs for RNAi by In Vitro Transcription
Abstract
This is a simple and widely used method for the preparation of dsRNA using a polymerase chain reaction (PCR) template-based, in vitro transcription reaction. The resulting dsRNA is then used to trigger RNAi in an appropriate cell or organism.
Footnotes
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From the Molecular Cloning collection, edited by Michael R. Green and Joseph Sambrook.
