PCR Buffer for DNA Template Preparation (10×)
| Reagent | Quantity (for 100 mL) | Final concentration (10×) |
|---|---|---|
| Tris-HCl (1 m, pH 8.3) | 10 mL | 100 mm |
| KCl (2 m) | 25 mL | 500 mm |
| MgCl2 (1 m) | 1.5 mL | 15 mm |
| Gelatin (2%, w/v) | 5 mL | 0.1% (w/v) |
| H2O | to 100 mL | |
| Store in 1-mL aliquots at −20°C. | ||
