Recipe

Egg Lysis Buffer (ELB-Sucrose)

Reagent Quantity (1 L) Final concentration
Sucrose  85.6 g    0.25 m
ELB salts (10×) 100 mL       1×
DTT (Bio-Rad 161-0611) 154 mg 0.154 mg/mL
Cycloheximide (Calbiochem 239763)  50 mg  0.05 mg/mL
Use ultrapure sucrose (≥99%; Invitrogen 15503-022). Adjust volume to 1 L with dH2O. Make fresh on the day of extract preparation.
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  1. doi:10.1101/pdb.rec104430 Cold Spring Harb Protoc 2019: pdb.rec104430- © 2019 Cold Spring Harbor Laboratory Press
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