Protocol

Preparation of siRNA Duplexes

  1. Phillip D. Zamore

Abstract

This protocol describes how to anneal synthetic sense and antisense siRNAs to form siRNA duplexes, as well as the analysis of siRNA duplexes using nondenaturing polyacrylamide gel electrophoresis.

Footnotes

  • From the Molecular Cloning collection, edited by Michael R. Green and Joseph Sambrook.

| Table of Contents

This Article

  1. doi:10.1101/pdb.prot097444 Cold Spring Harb Protoc 2019: pdb.prot097444- © 2019 Cold Spring Harbor Laboratory Press
  1. » Abstract
  2. Full Text
  3. Full Text (PDF)

Article Category

  1. Protocol

Personal Folder

  1. Save to Personal Folders

Updates/Comments

  1. Submit Updates/Comments
  2. No Updates/Comments published
  3. Alert me when Updates/Comments are published

Share

Current Issue

  1. January 2026, 2026 (1)
  1. Current Issue