Protocol

RNAi in Drosophila S2 Cells by siRNA Duplex or dsRNA Transfection

  1. Phillip D. Zamore

Abstract

This protocol describes an efficient method to trigger RNAi in Drosophila S2 cells by using transfection reagents to deliver siRNAs or dsRNAs.

Footnotes

  • From the Molecular Cloning collection, edited by Michael R. Green and Joseph Sambrook.

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  1. doi:10.1101/pdb.prot097485 Cold Spring Harb Protoc 2019: pdb.prot097485- © 2019 Cold Spring Harbor Laboratory Press
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