GST Binding Buffer
25 mm Tris-HCl, pH 7.5
30 mm MgCl2
40 mm NaCl
1 mm dithiothreitol
0.5% Nonidet P-40
complete, Mini Protease Inhibitor (1 tablet/10 mL; Roche 04693124001)
PhosStop Phosphatase Inhibitor (1 tablet/10 mL; Roche PHOSS-RO)
Prepare fresh. Keep on ice until use.
