Recipe

Normal Amphibian Medium (NAM) (10×)

1.1 m NaCl

20 mm sodium phosphate (pH 7.4)

20 mm KCl

10 mm Ca(NO3)2

10 mm MgSO4

10 mm NaHCO3

1 mm EDTA

Store at room temperature.

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  1. doi:10.1101/pdb.rec104976 Cold Spring Harb Protoc 2019: pdb.rec104976- © 2019 Cold Spring Harbor Laboratory Press
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