Recipe

1/20× NAM (Normal Amphibian Medium)

Reagent Volume
10× NAM salts solution   5 mL
0.1 m NaPO4, pH 7.5  20 mL
0.1 m NaHCO3 0.5 mL
Bring to 1 L with autoclaved dH2O. Prepare using sterile technique; do not resterilize this working solution. (This recipe was adapted from Slack 1984.)

References

  1. ↵
    Slack JMW. 1984. Regional biosynthetic markers in the early amphibian embryo. J Embryol Exp Morphol 80: 289–319.
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  1. doi:10.1101/pdb.rec105031 Cold Spring Harb Protoc 2019: pdb.rec105031- © 2019 Cold Spring Harbor Laboratory Press
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