Buffer A for DNase-seq
| Reagent | Final concentration |
|---|---|
| Tris-HCl (1 m, pH 8.0) | 15 mm |
| NaCl (5 m) | 15 mm |
| KCl (1 m) | 60 mm |
| EDTA (0.5 m [pH 8.0]) | 1 mm |
| EGTA (50 mm [pH 8.0]) | 0.5 mm |
| Spermine (500 mm) | 0.5 mm |
| Pefabloc SC PLUS (Roche 11873601001) (20 mg/mL) | 0.1 mg/mL |
| Dithiothreitol (DTT) (1 m) | 2 mm |
| Protease inhibitor tablet (cOmplete, Mini, EDTA-free; Roche 04693159001) | 1 tablet for 10 mL |
| Combine Tris-HCl, NaCl, KCl, EDTA, EGTA, and spermine. Store the buffer at 4°C. Immediately before use, add Pefabloc SC, DTT, and protease inhibitor. | |
