RNA Interference and Small RNA Analysis
Abstract
Small silencing RNAs have provided powerful reverse genetics tools and have opened new areas of research. This introduction describes the use of RNAi to suppress expression of individual genes for loss-of-function analysis. It also summarizes methods for measuring specific and global changes in small RNA expression, as well as methods to inhibit the function of individual endogenous small RNA species such as miRNAs.
Footnotes
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From the Molecular Cloning collection, edited by Michael R. Green and Joseph Sambrook.
