Adoptive Transfer of Fluorescently Labeled Immune Cells in Xenopus
- 1Department of Immunology Microbiology and Virology, University of Rochester, Medical Center, New York 14620
- ↵2Correspondence: Kunhyoe_rhoo{at}urmc.rochester.edu
Abstract
Adoptive cell transfer from inbred adult Xenopus to inbred tadpoles is a useful way to study the dissemination of immune cells or pathogen-infected immune cells in tadpoles. For example, Xenopus peritoneal leukocytes (PLs) can be readily infected by pathogens such as Frog virus 3 (FV3) and Mycobacterium marinum (M. marinum). By transferring fluorescently labeled, FV3-infected PLs into tadpoles, we observed infiltration of these cells into the tadpole's brain, which indicates that FV3-infected PLs can cross blood brain barrier. Taking advantage of tadpoles’ transparency, fluorescently labeled immune cells can be tracked in real time using fluorescence microscopy.
Footnotes
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From the Xenopus collection, edited by Hazel L. Sive.
