Recipe

Steinberg's Solution (1×)

3.4 g/L NaCl

0.05 g/L KCl

0.08 g/L Ca(NO3)2·4H2O

0.1025 g/L MgSO4

0.56 g/L Tris-HCl (pH 7.4)

10 mg/L phenol red

For convenience, prepare a 10× stock solution. Store the 10× stock solution for no longer than a few months at room temperature.

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  1. doi:10.1101/pdb.rec106336 Cold Spring Harb Protoc 2019: pdb.rec106336- © 2019 Cold Spring Harbor Laboratory Press

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