BWT (Bind & Wash & Tween) Buffer (2×)
| Reagent | Amount to add (for 50 mL) | Final concentration |
|---|---|---|
| Tris-HCl (1 m, pH 7.5) | 500 µL | 10 mm |
| NaCl (5 m) | 20 mL | 2 m |
| EDTA (0.5 m, pH 8.0) | 100 µL | 1 mm |
| Tween 20 (10%) | 1 mL | 0.2% |
| ddH2O (sterile) | 28.40 mL | |
| Store for up to 6 mo at room temperature. | ||
