Recipe

TTLoE Buffer

Reagent Amount to add (for 10 mL) Final concentration
Tris-HCl (1 m, pH 8.0)  50 µL  5 mm
Tween 20 (10%)  25 µL 0.025%
EDTA (0.5 m, pH 8.0)   2 µL 0.1 mm
ddH2O (sterile) 9.92 mL
Store for up to 6 mo at 4°C.
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  1. doi:10.1101/pdb.rec106468 Cold Spring Harb Protoc 2019: pdb.rec106468- © 2019 Cold Spring Harbor Laboratory Press
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