Cover image

A colorful collage of Xenopus laevis and Xenopus tropicalis spindles showing microtubules labeled with rhodamine tubulin and chromosomes with Hoechst DNA stain. Spindles were assembled in vitro using Xenopus egg extracts. In this issue, Romain Gibeaux and Rebecca Heald introduce methods for preparing and using cell-free Xenopus extracts to investigate cytoplasmic events (doi: 10.1101/pdb.top097048). Image courtesy of Romain Gibeaux and Rebecca Heald.