Calculating the Degradation Rate of Individual Proteins Using Xenopus Extract Systems
- Gary S. McDowell1,2,5 and
- Anna Philpott3,4,5
- 1Future of Research, Abington, Massachusetts 02351;
- 2Manylabs, San Francisco, California 94103;
- 3Department of Oncology, MRC/Hutchison Research Centre, Cambridge CB21XZ, United Kingdom;
- 4Wellcome Trust-Medical Research Centre Cambridge Stem Cell Institute, University of Cambridge, Cambridge CB21QR, United Kingdom
- ↵5Correspondence: ap113{at}cam.ac.uk; garymcdow{at}gmail.com
Abstract
The Xenopus extract system has been used extensively as a simple, quick, and robust method for assessing the stability of proteins against proteasomal degradation. In this protocol, methods are provided for assessing the half-life of in vitro translated radiolabeled proteins using Xenopus egg or embryo extracts.
Footnotes
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From the Xenopus collection, edited by Hazel L. Sive.
