Vosper Buffer
| Reagent | Quantity |
|---|---|
| Urea | 48.05 g |
| Tris (1 m, pH 7.4) | 10 mL |
| NaCl | 3.51 g |
| Imidazole | 0.136 g |
| IGEPAL (CA-630; Sigma-Aldrich) or Nonidet P40 | 1 mL |
| Make up to 100 mL with water. No sterilization is necessary. Store at room temperature and use on day of preparation. | |
