Recipe

Reducing Buffer Solution

Reagent Quantity
2× Laemmli sample buffer with β-mercaptoethanol , adjusted to pH 10 with NaOH  1 mL
β-mercaptoethanol  100 µL
Imidazole 7.49 mg
Total volume  1.1 mL
Prepare fresh on day of use. No sterilization is necessary. Store at room temperature.
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  1. doi:10.1101/pdb.rec104760 Cold Spring Harb Protoc 2019: pdb.rec104760- © 2019 Cold Spring Harbor Laboratory Press
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