Nuclear Purification Buffer (NPB)
10 mm Tris (pH 7.4)
40 mm NaCl
90 mm KCl
2 mm EDTA
0.5 mm EGTA
0.2 mm dithiothreitol (DTT)
0.5 mm phenylmethanesulfonyl fluoride (PMSF)
0.5 mm spermine (Sigma-Aldrich S3256)
0.25 mm spermidine (Sigma-Aldrich S2626)
1× Roche cOmplete Protease Inhibitor Cocktail (Sigma-Aldrich 11697498001)
Add DTT, PMSF, spermine, spermidine, and protease inhibitors to NPB immediately prior to use. Use at 4°C.
