Protocol

Synthesis of cDNA Probes from mRNA Using Random Oligonucleotide Primers

  1. Joseph Sambrook

Abstract

This protocol describes the generation of radiolabeled cDNA probes from poly(A)+ RNA in a random priming reaction. Probes of this type are used for differential screening of cDNA libraries.

Footnotes

  • From the Molecular Cloning collection, edited by Michael R. Green and Joseph Sambrook.

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  1. doi:10.1101/pdb.prot100636 Cold Spring Harb Protoc 2019: pdb.prot100636- © 2019 Cold Spring Harbor Laboratory Press
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