Recipe

aa-dUTP Mixture (50×)

Dissolve 1 mg of aminoallyl-dUTP (Sigma-Aldrich) with:

Reagent Quantity Final concentration
dATP (100 mm) 32.0 µL 12.5 mm
dGTP (100 mm) 32.0 µL 12.5 mm
dCTP (100 mm) 32.0 µL 12.5 mm
dTTP (100 mm) 12.7 µL   5 mm
dH2O 19.3 µL
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  1. doi:10.1101/pdb.rec096784 Cold Spring Harb Protoc 2019: pdb.rec096784- © 2019 Cold Spring Harbor Laboratory Press
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