Recipe

Second-Strand Synthesis Buffer (5×)

Reagent Final concentration
Tris–HCl (pH 6.9)  100 mm
MgCl2   23 mm
KCl  450 mm
β-NAD 0.75 mm
(NH4)2SO4   50 mm
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  1. doi:10.1101/pdb.rec096826 Cold Spring Harb Protoc 2019: pdb.rec096826- © 2019 Cold Spring Harbor Laboratory Press
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