Buffer D (Dialysis Buffer)
| Reagent | Volume per 1 L of solution (v/v) | Final concentration |
|---|---|---|
| HEPES-KOH (1 m, pH 7.9) | 20 mL | 20 mm |
| KCl (1 m) | 100 mL | 0.1 m |
| Glycerol | 200 mL | 20% |
| EDTA (0.5 m) | 400 µL | 0.2 mm |
| DTT (1 m) | 0.5 mL | 0.5 mm |
| PMSF (100 mm) | 5 mL | 0.5 mm |
| ddH2O | Add to 1 L | |
| Store at 4°C, and only add PMSF immediately before use. | ||
