3,3′,5-Triiodo-L-Thyronine Sodium Salt Solution (T₃; 5 nm)

• 1. Make a 0.01 m stock solution of T₃ by adding 100 mg of T₃ powder (Sigma-Aldrich; CAS 55-06-1) to 14.86 mL of 30% NaOH (prepared in Milli-Q [ultrapure] water). Vortex the 0.01 m stock solution well, aliquot, and store at −20°C.

• 2. Make a 0.0001 m intermediate solution of T₃ fresh every day by adding 5 µL of 0.01 m stock solution of T₃ to 495 µL of Evian water (use Evian water in a 75 cL glass bottle or another mineral water with reproducible quality).

  • Be careful that neither precipitate nor crystals are present in the T₃ stock solution.

• 3. Add 5 µL of 0.0001 m T₃ to 100 mL of Evian water to create a final 5 nm T₃ solution.

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