Recipe

aa-dNTP/Cy-dNTP Mixture (100×)

Reagent Final concentration
dATP 25 mm
dCTP 25 mm
dGTP 25 mm
dTTP 10 mm
Aminoallyl-dUTP or CyX-dUTP 15 mm
The ratio of aa-dUTP to dTTP can be altered or optimized.
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  1. doi:10.1101/pdb.rec096776 Cold Spring Harb Protoc 2019: pdb.rec096776- © 2019 Cold Spring Harbor Laboratory Press
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