Tween Solution (10%)
| Reagent concentration | Quantity (for 50 mL) | Final concentration |
|---|---|---|
| Tween 20 (50%) | 10 mL | 10%, w/v |
| HEPES (1 m, pH 8) | 1 mL | 20 mm |
| H2O | 39 mL | |
| Make a 50% solution of Tween 20 by diluting 1:1 with water. Combine 10 mL of 50% Tween 20, 1 mL of 1 m HEPES, and 39 mL of water to make a 50-mL stock solution. Sterilize by passing through a 0.22-µm filter. Store at 4°C. | ||
