Phosphate-Buffered Saline (PBS) for Immunoblotting (10×)
| Reagent | Quantity (for 1 L) | Final concentration |
|---|---|---|
| NaH2PO4 (monobasic) | 4.56 g | 38 mm |
| Na2HPO4 (dibasic) | 23 g | 0.162 m |
| NaCl | 80 g | 1.37 m |
| Ultrapure H2O | Up to 800 mL | |
| Adjust pH to 7.4 using HCl if necessary, then bring the volume up to 1 L. To prepare a 1× solution, mix one part of 10× PBS with nine parts of ultrapure H2O. | ||
