Transcription Buffer for Probe Preparation (10×)
| Reagent | Quantity (for 1 mL) | Final concentration |
|---|---|---|
| Tris-Cl (1 m, pH 7.5) | 400 µL | 400 mm |
| MgCl2 (1 m) | 60 µL | 60 mm |
| Spermidine HCl (100 mm) | 200 µL | 20 mm |
| NaCl (5 m) | 10 µL | 50 mm |
| Sterilize the 10× buffer by filtration and then store it in small aliquots at −20°C. Discard each aliquot after use. | ||
