Topic Introduction

In Vitro Transcription Systems

  1. Joseph Sambrook

Abstract

Virtually all in vitro transcription of DNA into RNA is performed with bacteriophage-encoded DNA-dependent RNA polymerases. These enzymes and their characteristics are introduced here.

Footnotes

  • From the Molecular Cloning collection, edited by Michael R. Green and Joseph Sambrook.

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  1. doi:10.1101/pdb.top100750 Cold Spring Harb Protoc 2020: pdb.top100750- © 2020 Cold Spring Harbor Laboratory Press
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