Lysis Buffer 1 (1×)
| Reagent | Volume per 100 mL of solution (v/v) | Final concentration |
|---|---|---|
| Tris-HCl (1 m, pH 7.5) | 5 mL | 50 mm |
| KCl (1 m) | 12 mL | 120 mm |
| EDTA (0.5 m) | 1 mL | 5 mm |
| Nonidet P-40 (10%) | 100 µL | 0.1% |
| Glycerol (50%) | 20 mL | 10% |
| ddH2O | Add to 100 mL. | |
| Store at 4°C. | ||
