Lysis Buffer 2 (1×)
| Reagent | Volume per 100 mL of solution (v/v) | Final concentration |
|---|---|---|
| Tris-HCl (1 m, pH 7.5) | 5 mL | 50 mm |
| NaCl (5 m) | 2 mL | 100 mm |
| EDTA (0.5 m) | 0.2 mL | 1 mm |
| ddH2O | Add to 100 mL | |
| PMSF (0.1 m) | 1 mL | 1 mm |
| Protease inhibitors (100×) | 1 mL | 1× |
| Phosphatase inhibitors (100×) | 1 mL | 1× |
| Store at 4°C. Add PMSF and protease and phosphatase inhibitors immediately before use. | ||
