Recipe

MEM Salts (10×; pH 7.4)

209.3 g MOPS

76.08 g EGTA

2.46 g MgSO4

Add to a 2-L beaker. Make up to 1 L with deionized H2O. Adjust pH to 7.4 using NaOH and sterilize by autoclaving. Store at 4°C protected from light (wrapped in foil).

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  1. doi:10.1101/pdb.rec106948 Cold Spring Harb Protoc 2020: pdb.rec106948- © 2020 Cold Spring Harbor Laboratory Press
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