Protocol

Animal Maintenance Systems: Xenopus tropicalis

  1. Marko E. Horb2
  1. National Xenopus Resource, Marine Biological Laboratory, Woods Hole, Massachusetts 02543, USA
  1. ↵2Correspondence: mhorb{at}mbl.edu

  • ↵1 Present address: Iwaki Aquatic, Holliston, Massachusetts 01746

Abstract

Modular recirculating animal aquaculture systems incorporate UV sterilization and biological, mechanical, and activated carbon filtration, creating a nearly self-contained stable housing environment for Xenopus tropicalis. Nonetheless, minimal water exchange is necessary to mitigate accumulation of metabolic waste, and regular weekly, monthly, and yearly maintenance is needed to ensure accurate and efficient operation. This protocol describes the methods for establishing a new recirculating system and the necessary maintenance, as well as water quality parameters, required for keeping Xenopus tropicalis.

Footnotes

  • From the Xenopus collection, edited by Hazel L. Sive.

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This Article

  1. Published in Advance May 13, 2020, doi: 10.1101/pdb.prot106146 Cold Spring Harb Protoc 2020: pdb.prot106146- © 2020 Cold Spring Harbor Laboratory Press
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