Recipe

Embryo Extraction Buffer

10 mm HEPES (pH 8.5)

2 mm MgCl2

1 mm EDTA

10 mm β-glycerophosphate

1 mm dithiothreitol (DTT)

Store for up to 1 wk at 4°C.

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  1. doi:10.1101/pdb.rec107185 Cold Spring Harb Protoc 2020: pdb.rec107185- © 2020 Cold Spring Harbor Laboratory Press
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