Frog Ringer
| Reagent | Amount (per L) | Final concentration |
|---|---|---|
| NaCl | 5.727 g | 98 mmol/L |
| KCl | 0.149 g | 2 mmol/L |
| CaCl2 dihydrate | 0.147 g | 1 mmol/L |
| MgCl2 hexahydrate | 0.407 g | 2 mmol/L |
| d-glucose | 0.900 g | 5 mmol/L |
| Sodium pyruvate | 0.550 g | 5 mmol/L |
| Hydroxyethyl piperazineethanesulfonic acid (HEPES) | 2.383 g | 10 mmol/L |
| Prepare in distilled H2O. Adjust the pH to 7.8 and confirm that the osmolarity is 230 mOsmol/L. Store for 1–2 wk at 4°C. | ||
