Production of Recombinant Adeno-Associated Viruses (rAAVs) by Transient Transfection
Abstract
The most commonly used method for production of recombinant adeno-associated virus (rAAVs) in research laboratories is by transient triple transfection of 293 cells with AAV cis and trans plasmids and an adenovirus helper plasmid. This protocol describes the processes required to prepare the transfected cell suspension for virus purification.
Footnotes
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From the Molecular Cloning collection, edited by Michael R. Green and Joseph Sambrook.
