FPLC Buffer B
| Reagent | Quantity (for 1 L) | Final concentration |
|---|---|---|
| Tris-HCl (1 m, pH 8.5) | 20 mL | 20 mm |
| NaCl (5 m) | 100 mL | 500 mm |
| H2O | to 1 L | |
| Adjust the pH of the final solution to 8.5. Pass through 0.22-µm filter and de-gas for 10 min before using in the FPLC system. | ||
