Iodixanol Solution (15%) (OptiPrep)
| Reagent | Quantity (for 100 mL) | Final concentration |
|---|---|---|
| Iodixanol stock | 25 mL | 15%, w/v |
| D-PBS (10×) | 10 mL | 1× |
| NaCl (5 m) | 20 mL | 1 m |
| MgCl2 (1 m) | 100 µL | 1 mm |
| KCl (1 m) | 250 µL | 2.5 mm |
| Phenol red (0.5%, w/v, in ethanol) | 150 µL | |
| H2O | To 100 mL |
