Recipe

Iodixanol Solution (25%) (OptiPrep)

Reagent Quantity (for 100 mL) Final concentration
Iodixanol stock   41.66 mL  25%, w/v
D-PBS (10×)     10 mL    1×
MgCl2 (1 m)    100 µL    1 mm
KCl (1 m)    250 µL  2.5 mm
Phenol red (0.5%, w/v, in ethanol)    200 µL
H2O To 100 mL
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  1. doi:10.1101/pdb.rec100156 Cold Spring Harb Protoc 2020: pdb.rec100156- © 2020 Cold Spring Harbor Laboratory Press
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