Recipe

Iodixanol Solution (60%) (OptiPrep)

Reagent Quantity (for 100 mL) Final concentration
Iodixanol stock   100 mL ∼60%, w/v
MgCl2 (1 m)    100 µL      1 mm
KCl (1 m)    250 µL    2.5 mm
Phenol red (0.5%, w/v, in ethanol)     50 µL
H2O To 100 mL
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  1. doi:10.1101/pdb.rec100172 Cold Spring Harb Protoc 2020: pdb.rec100172- © 2020 Cold Spring Harbor Laboratory Press
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