Protocol

Applying Tensile and Compressive Force to Xenopus Animal Cap Tissue

  1. Sarah Woolner1
  1. Wellcome Trust Centre for Cell-Matrix Research, Faculty of Biology, Medicine and Health, Manchester Academic Health Science Centre, University of Manchester, Oxford Road, Manchester M13 9PT, United Kingdom
  1. 1Correspondence: sarah.woolner{at}manchester.ac.uk

Abstract

Over many years, the Xenopus laevis embryo has provided a powerful model system to investigate how mechanical forces regulate cellular function. Here, we describe a system to apply reproducible tensile and compressive force to X. laevis animal cap tissue explants and to simultaneously assess cellular behavior using live confocal imaging.

Footnotes

  • From the Xenopus collection, edited by Hazel L. Sive.

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